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Image Search Results
Journal: Frontiers in Plant Science
Article Title: Overexpression of the PeaT1 Elicitor Gene from Alternaria tenuissima Improves Drought Tolerance in Rice Plants via Interaction with a Myo-Inositol Oxygenase
doi: 10.3389/fpls.2017.00970
Figure Lengend Snippet: PeaT1 interacts with OsMIOX. (A) Results of a yeast two-hybrid assay involving BD-PeaT1 and AD-OsMIOX. The transformed yeast cells were plated on SD/-Leu/-Trp medium (left) and SD/-Leu/-Trp/-His medium supplemented with 50 mM 3-AT (middle). The results of the X-gal assay are also provided (right). (B) Purified proteins stained with Coomassie Brilliant Blue. (C) A solution consisting of PeaT1-GST bound to the GST-binding resin was incubated with an equal volume of cell lysate containing OsMIOX-MBP or MBP. The binding of OsMIOX-MBP or MBP to the GST-binding resin before (Input) and after (Pull-down) washes was examined in a western blot involving the anti-MBP antibody. The same protein gel was used for a western blot involving the anti-GST antibody to verify that equal amounts of GST-PeaT1 were present in all protein mixtures. ∗ Represents the target protein band.
Article Snippet: The amylose resin for purifying MBP (New England Biolabs) and the
Techniques: Y2H Assay, Transformation Assay, Purification, Staining, Binding Assay, Incubation, Western Blot