gst-binding resin Search Results


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Promega gst-binding resin magnegsttm glutathione particles
Gst Binding Resin Magnegsttm Glutathione Particles, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA gst bind resin
Gst Bind Resin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA novagen gst•bind resin
Novagen Gst•Bind Resin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega gst-binding resin magnegst glutathione particles
Gst Binding Resin Magnegst Glutathione Particles, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA gst-binding resin for purifying gst
PeaT1 interacts with OsMIOX. (A) Results of a yeast two-hybrid assay involving BD-PeaT1 and AD-OsMIOX. The transformed yeast cells were plated on SD/-Leu/-Trp medium (left) and SD/-Leu/-Trp/-His medium supplemented with 50 mM 3-AT (middle). The results of the X-gal assay are also provided (right). (B) Purified proteins stained with Coomassie Brilliant Blue. (C) A solution consisting of <t>PeaT1-GST</t> bound to the GST-binding resin was incubated with an equal volume of cell lysate <t>containing</t> <t>OsMIOX-MBP</t> or MBP. The binding of OsMIOX-MBP or MBP to the GST-binding resin before (Input) and after (Pull-down) washes was examined in a western blot involving the anti-MBP antibody. The same protein gel was used for a western blot involving the anti-GST antibody to verify that equal amounts of GST-PeaT1 were present in all protein mixtures. ∗ Represents the target protein band.
Gst Binding Resin For Purifying Gst, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-binding resin for purifying gst/product/Merck KGaA
Average 90 stars, based on 1 article reviews
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Merck KGaA gst-bind resin-slurry
PeaT1 interacts with OsMIOX. (A) Results of a yeast two-hybrid assay involving BD-PeaT1 and AD-OsMIOX. The transformed yeast cells were plated on SD/-Leu/-Trp medium (left) and SD/-Leu/-Trp/-His medium supplemented with 50 mM 3-AT (middle). The results of the X-gal assay are also provided (right). (B) Purified proteins stained with Coomassie Brilliant Blue. (C) A solution consisting of <t>PeaT1-GST</t> bound to the GST-binding resin was incubated with an equal volume of cell lysate <t>containing</t> <t>OsMIOX-MBP</t> or MBP. The binding of OsMIOX-MBP or MBP to the GST-binding resin before (Input) and after (Pull-down) washes was examined in a western blot involving the anti-MBP antibody. The same protein gel was used for a western blot involving the anti-GST antibody to verify that equal amounts of GST-PeaT1 were present in all protein mixtures. ∗ Represents the target protein band.
Gst Bind Resin Slurry, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA gst-bind resin column
PeaT1 interacts with OsMIOX. (A) Results of a yeast two-hybrid assay involving BD-PeaT1 and AD-OsMIOX. The transformed yeast cells were plated on SD/-Leu/-Trp medium (left) and SD/-Leu/-Trp/-His medium supplemented with 50 mM 3-AT (middle). The results of the X-gal assay are also provided (right). (B) Purified proteins stained with Coomassie Brilliant Blue. (C) A solution consisting of <t>PeaT1-GST</t> bound to the GST-binding resin was incubated with an equal volume of cell lysate <t>containing</t> <t>OsMIOX-MBP</t> or MBP. The binding of OsMIOX-MBP or MBP to the GST-binding resin before (Input) and after (Pull-down) washes was examined in a western blot involving the anti-MBP antibody. The same protein gel was used for a western blot involving the anti-GST antibody to verify that equal amounts of GST-PeaT1 were present in all protein mixtures. ∗ Represents the target protein band.
Gst Bind Resin Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PeaT1 interacts with OsMIOX. (A) Results of a yeast two-hybrid assay involving BD-PeaT1 and AD-OsMIOX. The transformed yeast cells were plated on SD/-Leu/-Trp medium (left) and SD/-Leu/-Trp/-His medium supplemented with 50 mM 3-AT (middle). The results of the X-gal assay are also provided (right). (B) Purified proteins stained with Coomassie Brilliant Blue. (C) A solution consisting of PeaT1-GST bound to the GST-binding resin was incubated with an equal volume of cell lysate containing OsMIOX-MBP or MBP. The binding of OsMIOX-MBP or MBP to the GST-binding resin before (Input) and after (Pull-down) washes was examined in a western blot involving the anti-MBP antibody. The same protein gel was used for a western blot involving the anti-GST antibody to verify that equal amounts of GST-PeaT1 were present in all protein mixtures. ∗ Represents the target protein band.

Journal: Frontiers in Plant Science

Article Title: Overexpression of the PeaT1 Elicitor Gene from Alternaria tenuissima Improves Drought Tolerance in Rice Plants via Interaction with a Myo-Inositol Oxygenase

doi: 10.3389/fpls.2017.00970

Figure Lengend Snippet: PeaT1 interacts with OsMIOX. (A) Results of a yeast two-hybrid assay involving BD-PeaT1 and AD-OsMIOX. The transformed yeast cells were plated on SD/-Leu/-Trp medium (left) and SD/-Leu/-Trp/-His medium supplemented with 50 mM 3-AT (middle). The results of the X-gal assay are also provided (right). (B) Purified proteins stained with Coomassie Brilliant Blue. (C) A solution consisting of PeaT1-GST bound to the GST-binding resin was incubated with an equal volume of cell lysate containing OsMIOX-MBP or MBP. The binding of OsMIOX-MBP or MBP to the GST-binding resin before (Input) and after (Pull-down) washes was examined in a western blot involving the anti-MBP antibody. The same protein gel was used for a western blot involving the anti-GST antibody to verify that equal amounts of GST-PeaT1 were present in all protein mixtures. ∗ Represents the target protein band.

Article Snippet: The amylose resin for purifying MBP (New England Biolabs) and the GST-binding resin for purifying GST (Merck, Germany) were used to purify the fusion proteins and empty tags.

Techniques: Y2H Assay, Transformation Assay, Purification, Staining, Binding Assay, Incubation, Western Blot